Kinetics of in vivo bone deposition by bone marrow stromal cells within a resorbable porous calcium phosphate scaffold: An X‐ray computed microtomography study
Identifieur interne : 001C04 ( Main/Exploration ); précédent : 001C03; suivant : 001C05Kinetics of in vivo bone deposition by bone marrow stromal cells within a resorbable porous calcium phosphate scaffold: An X‐ray computed microtomography study
Auteurs : A. Papadimitropoulos [Italie] ; M. Mastrogiacomo ; F. Peyrin [France] ; E. Molinari [Italie] ; V. S. Komlev [Italie, Russie] ; F. Rustichelli [Italie] ; R. CanceddaSource :
- Biotechnology and Bioengineering [ 0006-3592 ] ; 2007-09-01.
Descripteurs français
- KwdFr :
- Animaux, Cellules cultivées, Cellules stromales mésenchymateuses (), Cellules stromales mésenchymateuses (cytologie), Cinétique, Différenciation cellulaire, Implant résorbable, Ingénierie tissulaire (), Matériaux biocompatibles (), Ostéoblastes (), Ostéoblastes (cytologie), Ostéogenèse (physiologie), Ovis, Phosphates de calcium (), Porosité, Régénération tissulaire guidée (), Souris, Souris nude, Substituts osseux (), Test de matériaux, Tomodensitométrie ().
- MESH :
- cytologie : Cellules stromales mésenchymateuses, Ostéoblastes.
- physiologie : Ostéogenèse.
- Animaux, Cellules cultivées, Cellules stromales mésenchymateuses, Cinétique, Différenciation cellulaire, Implant résorbable, Ingénierie tissulaire, Matériaux biocompatibles, Ostéoblastes, Ovis, Phosphates de calcium, Porosité, Régénération tissulaire guidée, Souris, Souris nude, Substituts osseux, Test de matériaux, Tomodensitométrie.
English descriptors
- KwdEn :
- Absorbable Implants, Animals, Biocompatible Materials (chemistry), Bone Substitutes (chemistry), Calcium Phosphates (chemistry), Cell Differentiation, Cells, Cultured, Guided Tissue Regeneration (methods), Kinetics, Materials Testing, Mesenchymal Stromal Cells (cytology), Mesenchymal Stromal Cells (radiography), Mice, Mice, Nude, Osteoblasts (cytology), Osteoblasts (radiography), Osteogenesis (physiology), Porosity, Sheep, Tissue Engineering (methods), Tomography, X-Ray Computed (methods), bone substitute, bone tissue engineering, synchrotron MicroCT.
- MESH :
- chemical , chemistry : Biocompatible Materials, Bone Substitutes, Calcium Phosphates.
- cytology : Mesenchymal Stromal Cells, Osteoblasts.
- methods : Guided Tissue Regeneration, Tissue Engineering, Tomography, X-Ray Computed.
- physiology : Osteogenesis.
- radiography : Mesenchymal Stromal Cells, Osteoblasts.
- Absorbable Implants, Animals, Cell Differentiation, Cells, Cultured, Kinetics, Materials Testing, Mice, Mice, Nude, Porosity, Sheep.
Abstract
Resorbable ceramic scaffolds based on Silicon stabilized tricalcium phosphate (Si‐TCP) were seeded with bone marrow stromal cells (BMSC) and ectopically implanted for 2, 4, and 6 months in immunodeficient mice. Qualitative and quantitative evaluation of the scaffold material was performed by X‐ray synchrotron radiation computed microtomography (microCT) with a spatial resolution lower than 5 µm. Unique to these experiments was that microCT data were first collected on the scaffolds before implantation and then on the same scaffolds after they were seeded with BMSC, implanted in the mice and rescued after different times. Volume fraction, mean thickness and thickness distribution were evaluated for both new bone and scaffold phases as a function of the implantation time. New bone thickness increased from week 8 to week 16. Data for the implanted scaffolds were compared with those derived from the analysis of the same scaffolds prior to implantation and with data derived from 100% hydroxyapatite (HA) scaffold treated and analyzed in the same way. At variance with findings with the 100% HA scaffolds a significant variation in the density of the different Si‐TCP scaffold regions in the pre‐ and post‐implantation samples was observed. In particular a post‐implantation decrease in the density of the scaffolds, together with major changes in the scaffold phase composition, was noticeable in areas adjacent to newly formed bone. Histology confirmed a better integration between new bone and scaffold in the Si‐TCP composites in comparison to 100% HA composites where new bone and scaffold phases remained well distinct. Biotechnol. Bioeng. 2007; 98: 271–281. © 2007 Wiley Periodicals, Inc.
Url:
DOI: 10.1002/bit.21418
Affiliations:
- France, Italie, Russie
- Auvergne-Rhône-Alpes, District fédéral central, Rhône-Alpes
- Grenoble, Lyon, Moscou
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Le document en format XML
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Bioeng.</title><idno type="ISSN">0006-3592</idno><idno type="eISSN">1097-0290</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2007-09-01">2007-09-01</date><biblScope unit="volume">98</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="271">271</biblScope><biblScope unit="page" to="281">281</biblScope></imprint><idno type="ISSN">0006-3592</idno></series><idno type="istex">7FF314ADE34913112F52CA2D606B0F294772AF0A</idno><idno type="DOI">10.1002/bit.21418</idno><idno type="ArticleID">BIT21418</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-3592</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Absorbable Implants</term><term>Animals</term><term>Biocompatible Materials (chemistry)</term><term>Bone Substitutes (chemistry)</term><term>Calcium Phosphates (chemistry)</term><term>Cell Differentiation</term><term>Cells, Cultured</term><term>Guided Tissue Regeneration (methods)</term><term>Kinetics</term><term>Materials Testing</term><term>Mesenchymal Stromal Cells (cytology)</term><term>Mesenchymal Stromal Cells (radiography)</term><term>Mice</term><term>Mice, Nude</term><term>Osteoblasts (cytology)</term><term>Osteoblasts (radiography)</term><term>Osteogenesis (physiology)</term><term>Porosity</term><term>Sheep</term><term>Tissue Engineering (methods)</term><term>Tomography, X-Ray Computed (methods)</term><term>bone substitute</term><term>bone tissue engineering</term><term>synchrotron MicroCT</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Cellules stromales mésenchymateuses ()</term><term>Cellules stromales mésenchymateuses (cytologie)</term><term>Cinétique</term><term>Différenciation cellulaire</term><term>Implant résorbable</term><term>Ingénierie tissulaire ()</term><term>Matériaux biocompatibles ()</term><term>Ostéoblastes ()</term><term>Ostéoblastes (cytologie)</term><term>Ostéogenèse (physiologie)</term><term>Ovis</term><term>Phosphates de calcium ()</term><term>Porosité</term><term>Régénération tissulaire guidée ()</term><term>Souris</term><term>Souris nude</term><term>Substituts osseux ()</term><term>Test de matériaux</term><term>Tomodensitométrie ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Biocompatible Materials</term><term>Bone Substitutes</term><term>Calcium Phosphates</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules stromales mésenchymateuses</term><term>Ostéoblastes</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Mesenchymal Stromal Cells</term><term>Osteoblasts</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Guided Tissue Regeneration</term><term>Tissue Engineering</term><term>Tomography, X-Ray Computed</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Ostéogenèse</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Osteogenesis</term></keywords><keywords scheme="MESH" qualifier="radiography" xml:lang="en"><term>Mesenchymal Stromal Cells</term><term>Osteoblasts</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Absorbable Implants</term><term>Animals</term><term>Cell Differentiation</term><term>Cells, Cultured</term><term>Kinetics</term><term>Materials Testing</term><term>Mice</term><term>Mice, Nude</term><term>Porosity</term><term>Sheep</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Cellules stromales mésenchymateuses</term><term>Cinétique</term><term>Différenciation cellulaire</term><term>Implant résorbable</term><term>Ingénierie tissulaire</term><term>Matériaux biocompatibles</term><term>Ostéoblastes</term><term>Ovis</term><term>Phosphates de calcium</term><term>Porosité</term><term>Régénération tissulaire guidée</term><term>Souris</term><term>Souris nude</term><term>Substituts osseux</term><term>Test de matériaux</term><term>Tomodensitométrie</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Resorbable ceramic scaffolds based on Silicon stabilized tricalcium phosphate (Si‐TCP) were seeded with bone marrow stromal cells (BMSC) and ectopically implanted for 2, 4, and 6 months in immunodeficient mice. Qualitative and quantitative evaluation of the scaffold material was performed by X‐ray synchrotron radiation computed microtomography (microCT) with a spatial resolution lower than 5 µm. Unique to these experiments was that microCT data were first collected on the scaffolds before implantation and then on the same scaffolds after they were seeded with BMSC, implanted in the mice and rescued after different times. Volume fraction, mean thickness and thickness distribution were evaluated for both new bone and scaffold phases as a function of the implantation time. New bone thickness increased from week 8 to week 16. Data for the implanted scaffolds were compared with those derived from the analysis of the same scaffolds prior to implantation and with data derived from 100% hydroxyapatite (HA) scaffold treated and analyzed in the same way. At variance with findings with the 100% HA scaffolds a significant variation in the density of the different Si‐TCP scaffold regions in the pre‐ and post‐implantation samples was observed. In particular a post‐implantation decrease in the density of the scaffolds, together with major changes in the scaffold phase composition, was noticeable in areas adjacent to newly formed bone. Histology confirmed a better integration between new bone and scaffold in the Si‐TCP composites in comparison to 100% HA composites where new bone and scaffold phases remained well distinct. Biotechnol. Bioeng. 2007; 98: 271–281. © 2007 Wiley Periodicals, Inc.</div></front></TEI><affiliations><list><country><li>France</li><li>Italie</li><li>Russie</li></country><region><li>Auvergne-Rhône-Alpes</li><li>District fédéral central</li><li>Rhône-Alpes</li></region><settlement><li>Grenoble</li><li>Lyon</li><li>Moscou</li></settlement></list><tree><noCountry><name sortKey="Cancedda, R" sort="Cancedda, R" uniqKey="Cancedda R" first="R." last="Cancedda">R. Cancedda</name><name sortKey="Mastrogiacomo, M" sort="Mastrogiacomo, M" uniqKey="Mastrogiacomo M" first="M." last="Mastrogiacomo">M. Mastrogiacomo</name></noCountry><country name="Italie"><noRegion><name sortKey="Papadimitropoulos, A" sort="Papadimitropoulos, A" uniqKey="Papadimitropoulos A" first="A." last="Papadimitropoulos">A. Papadimitropoulos</name></noRegion><name sortKey="Komlev, V S" sort="Komlev, V S" uniqKey="Komlev V" first="V. S." last="Komlev">V. S. Komlev</name><name sortKey="Molinari, E" sort="Molinari, E" uniqKey="Molinari E" first="E." last="Molinari">E. Molinari</name><name sortKey="Rustichelli, F" sort="Rustichelli, F" uniqKey="Rustichelli F" first="F." last="Rustichelli">F. Rustichelli</name></country><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Peyrin, F" sort="Peyrin, F" uniqKey="Peyrin F" first="F." last="Peyrin">F. Peyrin</name></region><name sortKey="Peyrin, F" sort="Peyrin, F" uniqKey="Peyrin F" first="F." last="Peyrin">F. Peyrin</name></country><country name="Russie"><region name="District fédéral central"><name sortKey="Komlev, V S" sort="Komlev, V S" uniqKey="Komlev V" first="V. S." last="Komlev">V. S. Komlev</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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